Webb10 apr. 2024 · Selective macroautophagy/autophagy maintains cellular homeostasis through the lysosomal degradation of specific cellular proteins or organelles. The pro-survival effect of selective autophagy has been well-characterized, but the mechanism by which it drives cell death is still poorly understood. Here, we use a quantitative proteomic … http://themakelearningfun.com/wet-transfer-buffer-protocol
Scilit Article - Identification of HPCAL1 as a specific autophagy ...
WebbThermal shift assays Screen for small molecule drug candidate compounds that increase the stability of your target protein, indicating an interaction Self-association determination Determine if a protein is more likely to self-associate, and therefore become viscous or aggregated Chemical stability assessment WebbThermal shift assays utilize differential scanning fluorimetry (DSF) to measure the thermal stability of a target protein and the increase in protein melting temperature upon the binding of a stabilizing agent. It is useful for identifying ligands, buffer conditions, and cofactors affecting protein stability. arti invasif adalah
Design of the SARS-CoV-2 RBD vaccine antigen improves …
Webb20 mars 2024 · Protein thermal shift assays were performed with 3 μg (A) or 5 μg purified protein (B). 5 μL buffer stock solution and 2.5 μL Sypro® Orange (final concentration: … WebbThe objectives of this approach are to improve the protective efficacy of a given antigen by (i) focusing the immune response toward potently neutralizing antibody epitopes on the antigen; (ii) reducing or eliminating immune responses to poorly neutralizing and/or immunodominant epitopes within the antigen; (iii) optimizing the thermal stability … Webboptimal buffer, pH and ionic strength formulations that promote protein solubility and stability. Features • Compatible with ThermoFluor (Differential Scanning Fluorimetry, DSF, Protein Thermal Shift) and Dynamic Light Scattering assays • Formulation of 12 unique buffers, pH 4.5 - 9.5, versus 8 levels of ionic strength arti iom dalam perusahaan