Injected retroorbitally

Author: rbkt

August undefined, 2024

WebbThis retro-orbital (RO) injection technique has been successfully employed in both the injection of drugs in the adult fish as well as transplantation of whole kidney marrow … Webb6 apr. 2024 · For xenograft transplantation, 2 × 10 6 DNMT3A-KO K562 cells were injected intravenously into NSG mice. Mice were bled retro-orbitally to check for the percentage of hCD45, 2 weeks post engraftment.

Rapid tolerization of virus-activated tumor-specific CD8 - PNAS

Webb1 aug. 2000 · A total of 50 000 nucleated cells were retroorbitally injected into lethally irradiated congenic mice. After 8 days, spleens were isolated from reconstituted animals, and individual nodules were microdissected and placed in a proteinase K lysis solution for overnight digestion. Webb2 sep. 2008 · ( A) TRAMP and TRP-SIY mice were injected with naïve 2C cells and infected with WSN-SIY as in Fig. 2. Fourteen days later, cells from various tissues were incubated with SIY peptide and analyzed for cytokine expression. TNFα versus IFNγ staining profiles are shown for Thy1.1 + CD8 + 2C cells. bob and doge steam

National Center for Biotechnology Information

Webb5 jan. 2024 · Lethally irradiated CD45.1 recipient mice (split doses of 6 Gy separated by 4 hours; total body irradiation, cesium source) were injected retroorbitally with a 1:1 mixture of 1 × 10 6 wild-type CD45.1 whole bone marrow competitor cells and either 1 × 10 6 wild-type or Sin3BF/F vav1-cre+ CD45.2 whole bone marrow test cells. Webb20 apr. 2011 · Retro-orbital injections may seem aesthetically distasteful, but we argue that this easily mastered technique is ultimately more humane than alternative … WebbOffice of the Senior Vice President for Research 304 Old Main University Park, Pennsylvania 16802 OSVPR Phone: 814-863-9580 Email: [email protected] IRB Office Phone: 814-865-1775 bob and dean mcnett

Retro-orbital injections in mice Lab Animal - Nature

Injected retroorbitally

(PDF) Retro-orbital Injection in Adult Zebrafish - ResearchGate

Webbdecreases serum iron. 129S6/SvEvTac mice were injected retroorbitally with 1 mg/kg BMP-2 (n = 8) or an equal volume of vehicle alone (n = 7). Four hours after injection, blood and livers were harvested. (A) Total mRNA was isolated from livers and analyzed by quantitative real-time RT-PCR for hepcidin mRNA expression relative to expression of … Webb7 maj 2009 · A total of 2 million bone marrow cells were mixed at a 1:1 ratio and injected retroorbitally into lethally irradiated wild-type mice (Ly5.1). Recipient mice were conditioned with 1000 cGy from a 137 Cesium source at a rate of approximately 95 cGy/minute before transplantation.

Did you know?

Webb11 apr. 2016 · Direct bone marrow (BM) injection has been proposed as a strategy to bypass homing inefficiencies associated with intravenous (IV) hematopoietic stem … Webb系统性注射方法常见的有尾静脉注射，腹腔注射；原位注射即局部注射，根据需要在不同部位进行注射，比如脑立体定位注射。我们经常在文献中会看到如下六种常用的基本注 …

Webb26 mars 2015 · After 6 weeks, peripheral blood from recipient mice was obtained by retroorbital or tail vein bleeding, subjected to red blood cell lysis, and stained with APC-conjugated CD45.1 (eBioscience) and fluorescein isothiocyanate-conjugated CD45.2 (BD Biosciences) antibodies to verify donor cell engraftment by flow cytometric analysis. http://www.ichacha.net/retro-orbitally.html

Webb7 aug. 2014 · For transplantation, 8- to 10-week-old NSG mice were irradiated at 230 cGy using a cesium source and injected retro-orbitally with freshly transduced CD34 + cells immediately after the 12-hour transduction period, within 24 hours of irradiation. Webb3. Inject into the retro-bulbar sinus. 4. Remove needle gently to avoid injury to the eye. 5. Apply mild pressure to the injection site with a gauze sponge. Agents: This …

Webb1 maj 2011 · Retro-orbital injection is extensively used as an alternative for intravascular inoculation [105] as this method is less stressful for the animal [104]. This procedure is used particularly for...

Webbretro-orbitally 中文是什么意思发音: "retro-orbitally"怎么读中文翻译手机版 [网络] 复古轨道 "retro" 中文翻译 : n. (老式服装、音乐等的)重新流行，复旧，怀旧。 "retro-" 中文翻译 : … bob and dawn davis photographyWebb15 maj 1997 · Recently, investigators have sought ways to drive adult bone marrow–derived stem cells out of the resting state and into self-renewing cell divisions to facilitate transplantation, as well as to allow the incorporation of retroviral DNA. 1, 12-14 Treatment with 5-FU is commonly used to force murine stem cells into cycle, since they … bob and dean\u0027s twisted tunesWebb29 dec. 2024 · Retroorbital injections were performed behind the right eye. Approximately 50 microliters of sterile balanced salt solution (BSS) containing 10 11 of AAV particles … bob and don\u0027s plumbing lincoln ne