WebItem DharmaFECT 2 Transfection Reagent (2 x 10 mL) Company Horizon Discovery; Catalog Number T-2002-07A; This product is no longer available on Biocompare. … WebNov 9, 2016 · Cells were transfected with 100 nM siRNA against SCD1 (SMARTpool reagent; Dharmacon, Chicago, IL, USA) or control siRNA (nontargeting siRNA; Dharmacon) using DharmaFECT 4 transfection reagent (Dharmacon), and incubated for 24 h in 0.2% FCS-containing DMEM. Following transfection, the cells were starved for 24 h, treated …
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WebMar 8, 2024 · 2 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Ege University, Bornova, Izmir, Turkey. ... (Dharmafect 2). After verifying the efficacy of siEphA2-loaded nanoparticles, we further evaluated a potential combination with a histone lysine demethylase inhibitor, JIB-04. Silencing EphA2 by siEphA2-loaded DDAB-cSLN … WebBe the first to review this product. Efficient siRNA or microRNA transfection. To attain efficient and reliable siRNA or microRNA transfection, we offer DharmaFECT … oracle bacon
Reverse Transfection (RTF) siRNA Libraries
WebNov 10, 2011 · Cells grown on 6-well plates to 40% confluence were transfected with 50-100nM siRNA and 3-4 μL DharmaFECT 2 reagent. For all assays, pooled transfected cells were equally divided to ensure the identical cell populations, and cell starvation commenced 56 hours after transfection. All experiments were repeated a minimum of 3 times. Web• For final volumes of DharmaFECT Transfection Reagent that are different than those described in Table 4, please adjust the volumes of stock DharmaFECT Transfection Reagent and cell culture medium accordingly. • The Rehydration Solution may be stored under sterile conditions at room temperature for up to 2 hours prior to use. 3. WebLung cancer cells were transfected with USP14 siRNA (siUSP14#1 and siUSP14#2) or nonspecific siRNA (siNC) (all from Shanghai GenePharma Co., Ltd, China) using DharmaFECT one siRNA infection reagent (Thermo Fisher Scientific). The siRNA sequences were: siUSP14#1: GACAGAAAGUUAUGGUGAAAG; siUSP14#2: … oracle backup mode check