WebApr 11, 2024 · To identify transcription factors binding to the -370∼-118 region of TREM2 promoter, we first performed DNA pull-down assay using PCR-produced and biotinylated bait fragments -370∼+33 and -118∼+33. Proteins pulled-down from BV2 nuclear extracts and stained by Coomassie blue were excised from the gel and subjected to mass … WebBiotin Pull-Down and Preparation for Illumina Sequencing Perform all the following steps in low-bind tubes. 38) Prepare for biotin pull-down by washing 150μl of 10mg/ml Dynabeads MyOne Streptavidin T1 beads (Life technologies, 65602) with 400μl of 1X Tween Washing Buffer (1X TWB: 5mM Tris-HCl (pH 7.5); 0.5mM EDTA; 1M
Pierce™ Pull-Down Biotinylated Protein:Protein Interaction Kit
WebBiotin pull-down – purifies protein interactors of any biotinylated protein or biotin-tagged ligand; Complete kit – provides all components and detailed protocol for purifying protein:protein interactions; No special equipment … WebJul 4, 2024 · National Center for Biotechnology Information dr. neeraj desai il
Using TTchem-seq for profiling nascent transcription …
WebThe SAPA assay takes advantage of an extremely high binding affinity of biotin to avidin (Kd = 10-15 M), which ... In our initial experiments, we compared the pull-down by streptavidin-conjugated agarose vs plain agarose beads and found that plain beads did not pull down biotinylated DNA-protein complexes. This control may be unnecessary for ... WebJan 8, 2024 · Finally, we use yeast RNA spike-in for global normalization and to control for equal biotin tagging, 4SU pull-down and library preparation between samples (as discussed below). WebThe detailed is mixture of target DNA and biotin labeled probe in H2O, 95degree 5min followed by 37degree 5min for DNA binding, Rotation at RT 1 hr for pulling down. For the second time, I used 1X ... dr neeraj dokania