Biotin-coupled mirna capture

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August undefined, 2024

WebMiR-4306 response elements were predicted by bioinformatics algorithm and identified using dual-luciferase reporter, RNA immunoprecipitation, and biotin-coupled miRNA capture assays. Results CircPDE4D was markedly downregulated in OA cartilages and interleukin (IL)-1β-stressed human normal chondrocytes (HNC). WebFeb 19, 2024 · Provided herein are methods of identifying the spatial location of a nucleic acid in a biological sample. In some embodiments, the methods employ a template switching oligonucleotide. In some embodiments, the methods extend the capture probe to create a complementary DNA (cDNA) molecule of a capture analyte and produce second strand …

Hsa_circRNA_0088036 acts as a ceRNA to promote bladder cancer …

WebAug 7, 2024 · The capture probe, 35 to 50 base-long and coupled to biotin on its 3′ end, binds the 5′ half of the miRNA. The detection probe is composed of a 3′ end complementary to the miRNA and a chain of fluorescently labeled RNA sequences, forming a fluorescent barcode associated with the target miRNA. WebMay 15, 2024 · They found that miR-193a-3p functioned as a tumor suppressor in PCa cells, through bioinformatic prediction and biotin-coupled miRNA capture studies. Functional studies showed that miR-193a-3p reduced MCL1 expression, to suppress proliferation, migration, and invasion in cancer cells . how from infancy you have known

Frontiers Circular RNA HIPK3: A Key Circular RNA in a Variety of ...

WebJan 31, 2024 · The biotin-coupled RNA complex was pull-downed by incubating the cell lysates with streptavidin magnetic beads (Life Technologies, USA) for another 4 h. The … WebNov 9, 2024 · On the contrary, some studies argued that biotin moiety when attached to the 3′ end of the miRNA impede its association with AGO2. 41 Hence, biotin-labelled miRNA was not co-IPed with AGO2. 28 The negative result could be due to low transfection efficiency or post-translational modification of AGO2 that can hamper miRNA-AGO2 … WebFeb 10, 2024 · Biotin-coupled miRNA capture. The 3′ end biotinylated miR-578 or control mimic RNA (RiboBio) was transfected into RF/6A cells for 24 h at the concentration of 30 nM. The biotin-conjugated RNA complex was pulled down by incubating the cell lysates with streptavidin-coated magnetic beads (Life Technologies). The amount of cZBTB44 in … how frost free hydrant works

Circular RNA-ZBTB44 regulates the development of choroidal ...

Circ‐TCF4.85 silencing inhibits cancer progression through …

WebFeb 27, 2013 · Biotin-coupled miRNA capture Biotin-labelled miRNAs were transfected into HEK293 cells at a final concentration of 20 nM. Forty-eight hours after transfection, cells were collected and treated as ... WebJan 15, 2024 · Biotin-coupled circRNA and miRNA capture. Biotin-coupled miRNA and circRNA pull-down assays were performed as described previously [11, 12]. Briefly, 3′ end biotinylated circRNA-ACAP2 (digoxin-5′-TCACACAGGCAGCTTTGGAAGAAG-3′-Digoxin; RiboBio, Guangzhou, China) was transfected into SW480 cells at a final concentration of … how frogs make noiseWebOct 23, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed as described previously by … how frogs croak

"WebAug 17, 2024 · To validate the interactions between circCDYL and its targets, we performed RNA fluorescent in situ hybridization, biotin-coupled miRNA capture assay and biotin-coupled probe pull-down assay. Tight … " - Biotin-coupled mirna capture

Biotin-coupled mirna capture

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WebNov 23, 2024 · Biotin-coupled miRNA capture, fluorescence in situ hybridization and luciferase reporter assays were conducted to confirm the relationship between circMMP9 and miR-124. In this study, we screened differentially expressed circRNAs and identified circMMP9 in GBM. We found that circMMP9 acted as an oncogene, was upregulated in … WebJun 19, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed as previously described 11. Briefly, 3′-end biotinylated miR-587 mimics (Ribio, Guangzhou, China) were ...

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WebDec 20, 2024 · Biotin-coupled miRNA capture. For biotin-coupled capture, approximately 5 × 10 6 BCa cells (T24 and 253 J-BV) were transfected with biotin-miR135a-5p or negative control (NC) by … WebMay 26, 2024 · We conducted biotin-coupled miRNA capture and FISH to confirm the sponge effect of Cdr1as. Biotin-coupled miR-1270 analogously captured more Cdr1as than biotin-coupled NC. This phenomenon indicates that miR-1270 could bind to Cdr1as (Fig. 3G). PCR experiments were conducted to find out the relative abundance of miR-1270 in …

WebIn the biotin-coupled miRNA capture assay, the direct interaction between HIPK3 and miR-194 was verified. While upregulating HIPK3 promoted NSCLC cell proliferation, co-transfection with both HIPK3 and miR-194 abrogated the tumor-promoting effects of HIPK3. Altogether, these data corroborated that HIPK3 and miR-149 exert opposing roles in … WebJun 21, 2024 · In addition, luciferase reporter, biotin‑coupled miRNA capture and RNA immunoprecipitation assays revealed that circZYG11B could regulate RGC function through circZYG11B/microRNA‑620/PTEN signaling. Clinically, RT‑qPCR assays demonstrated that circZYG11B expression was markedly increased in the aqueous humor of patients with …

WebNov 17, 2024 · In addition, a biotin-coupled miRNA capture assay was performed using a biotin-coupled miR-185-3p probe, followed by qRT-PCR. It was observed a fourfold enrichment of circ_0008494 in the miR … WebApr 8, 2024 · Subsequently, a biotin-coupled miRNA capture assay was performed to detect the sponge effect between hsa_circRNA_0088036 and miR-140-3p. BCa cells transfected with oehsa_circRNA_0088036 were also ...

WebThe intracellular transfer of circEDIL3 by SMSCs-Exos may be a potential novel therapeutic strategy for Rheumatoid Arthritis.

WebMay 31, 2024 · The biotin-coupled miRNA capture assay was performed as previously described. 17 In brief, miR-206 mimics and miRNA negative control were labeled with biotin at the 3′ end and transiently transfected into LoVo and HCT116 cells. After 24 hrs of culture, the cells were harvested and lysed in a lysis buffer, followed by incubation with ... how fromsoft treat there employeesWebJul 21, 2024 · Biotin-coupled miRNA capture. Biotin-coupled miRNA pulldown assay was performed as described previously [27, 28]. In brief, 3′-end biotinylated miR-1-3p mimics (GenePharma) were transfected into YTS-1 cells using GP-transfect Mate reagent (GenePharma). Cells were lysed with lysis buffer ... how frosted mini wheats are madeWebDec 18, 2024 · Biotin-coupled miRNA capture The 3′ end biotinylated miRNA mimics or control RNA (Ribio, Guangzhou, China) were transfected into ccRCC cells for 48 h … highest cash back casino racebookWebJul 19, 2024 · Bioinformatic analysis was utilized to predict potential miRNA target sites, and biotin-coupled miRNA capture, biotin-coupled probe pull-down assay, and RNA … highest cash back cardWebJul 13, 2024 · 2.13 Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was carried out as previously described. 17, 18 Briefly, the 3′-end biotinylated miR-RNA mimic or control biotin-RNA (RiboBio) was transfected into SPCA1 cells at a final concentration of 20 nmol/L for 1 day. The biotin-coupled RNA complex was pulled … highest cash back card in canadaWebJun 25, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed using previously reported method 8. Briefly, the 3′-end biotinylated … highest cash back cards on all purchasesWebFeb 27, 2013 · Biotin-coupled miRNA capture Biotin-labelled miRNAs were transfected into HEK293 cells at a final concentration of 20 nM. Forty-eight hours after transfection, cells were collected and treated as ... highest cash back credit cards on everything